MOLECULAR IDENTIFICATION AND PHYLOGENETIC-TREE ANALYSIS OF MONIEZIA SPECIES FROM SHEEP IN AL-DIWANIYAH CITY
DOI:
https://doi.org/10.26842/binhm.7.2018.15.2.0131Keywords:
Cestoda, Moniezia, PCR, Phylogeny, Sheep.Abstract
The present study was performed to detect the molecular and the phylogenetic identification of species that belonging to the genus of Moniezia Blanchard, 1891 which affected intestines of sheep in Al-Diwaniyah city, Iraq; fifty intestine samples were sought for the infestation of Moniezia spp. from the city slaughterhouse from 1 October to 30 November 2017, this tapeworm was found to infest the intestines of 13 sheep.
For morphological identify the genus of this tapeworm, eggs from one gravid proglottid of the thirteen worms were examined, polymerase chain reaction (PCR) and the PCR-product-based sequencing were applied on 4 Moniezia tapeworms targeting a specific region of the 18S rRNA gene.
The sequencing has shown 2 species of Moniezia, SP1 and SP2 ,these two species revealed close matching on the phylogenetic tree to an according to the current study findings, Moniezia spp. affect on sheep in the city of Al-Diwaniyah, Iraq, these findings give interesting information about the evolution history of this worm in the studied city.
Downloads
References
Br¬usca, R. and Br¬usca, G. 1990. In¬ver¬te¬brates. Sun¬der¬land, M. A.: Sin¬auer As¬so¬ci¬ates, 636 pp.
Chilton, N., O'Callaghan, M., Bev¬eridge, I. and An¬drews, R. 2007. Ge¬netic mark¬ers to dis¬tin-guish Moniezia ex¬pansa from M. bene¬deni. Par¬a¬sitol¬ogy Re¬search, 100: 1187.
Denegri, G., Bernadina, W., Perez-Serrano, J. and Rodriguez-Caabeiro, F. 1998. Anoplocephalid cestodes of veterinary and medical significance: a review. Folia Parasitologica, 45(1): 1-8.
Diop, G., Yanagida, T., Hailemariam, Z., Menkir, S., Nakao, M., Sako, Y., Tidiane Ba, C. and Ito, A. 2015. Genetic characterization of Moniezia species in Senegal and Ethiopia. Parasitology International, 64(5): 256-260.
El-Shazly, A. M., Morsy, T. A. and Dawoud, H. A. 2004. Human Monieziasis expansa: the first Egyptian parastic zoonosis. Journal of the Egyptian Society Parasitology, 34 (2): 380–381.
Fadl, S. R., Kalef, D. A. and Abbas, S. M. 2011. Prevalence of parasitic infection in Sheep From different Regions in Baghdad. The Iraqi Journal of Veterinary Medicine, 1:204-209.
Gómez-Puerta, D. 2008. Occurrence of Moniezia expansa in dometic pig. Veterinary Parasitology, 33: 191-194.
Mazyad, S. A. M. and El-Nemr, H. I. 2002. The endoparasites of sheep and goats, and shepherd in North Sinai Governorate, Egypt. Journal of the Egyptian Society Parasitology, 32(1):119-126.
Mel¬horn, H. 2001. En¬cy¬clo¬pe¬dic ref¬er¬ence of parasitology. Berlin, Springer,1062 pp.
Nguyen, T. D., Le, Q. D., Huynh, V.V., Nguyen, S. T., Nguyen, T. V. and Vu-Khac, H. 2012. The development of PCR methodology for the identification of species of the tapeworm Moniezia from cattle, goats and sheep in central Vietnam. Journal of Helminthology, 86(4): 426-429.
Ohtori, M., Aoki, M., and Itagaki, T. 2015. Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomal RNA among three Moniezia species isolated from ruminants in Japan. The Journal of Veterinary Medical Sciences, 77(1): 105-107.
Rahif, R. H. 1998. The modification in the preparation of classical Carmen stain and there technique used for staining of Platyhelminthes (Cestodes and Trematod). The Veterinarian, 8(2):1-8.
Saitou, N., and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406–25.
Soulsby, E. J. L. 1982. Helminths, arthropods and protozoa of domesticated animals. 7th ed. London, BailliereTindall, 329 pp.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0., Molecular Biology and Evolution, 30: 2725–2729.
Wickström, L. M., Haukisalmi, V., Varis, S., Hantula, J. and Henttonen, H. 2005. Molecular Phylogeny and Systematics of Anoplocephaline Cestodes in Rodents and Lagomorphs. Systematic Parasitology, 62(2): 83-99.
Wymann, M. N. 2008. Gastrointestinal parasite egg excretion in young calves in periurban livestock production in Mali. Research in Veterinary Science, 84(2):225-231.
