MORPHOLOGICAL AND MOLECULAR STUDIES OF KAIS KINGFISH CYPRINION KAIS HECKEL, 1843 (PISCIES, CYPRINIFORMES, CYPRINIDAE) FROM THE MIDDLE OF IRAQ

Authors

  • Hind Dyia Hadi Iraq Natural History Research Center and Museum, University of Baghdad, Baghdad, Iraq
  • Abed Hassan Baraaj Department of Biology, College of Science, Baghdad University, Iraq.
  • Atheer Hussain Ali Department of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, Iraq.

DOI:

https://doi.org/10.26842/binhm.7.2023.17.4.0611

Keywords:

Cyprinidae, Cytochrome, Fishes, Morphology, Tigris River.

Abstract

Cyprinidae species are the most abundant and widely distributed fish species in the inland waters of Iraq. Cyprinids are complex species, and it is difficult to identify them on the basis of morphology. Thus, the morphological characteristics must be achieved and confirmed by molecular analysis. Twenty specimens of Cyprinion kais Heckel, 1843 (Piscies, Cypriniformes, Cyprinidae) were collected from two localities at Tigris River in the middle of Iraq: five specimens from Al-Tharthar Lake, Saladin Province, and 15 specimens from Al- Zubaydiyah sub-district, Wasit Province.
The DNA sequences of C. kais were done using the mitochondrial DNA cytochrome b (cytb) gene. After analysis, the sequences were compared with sequences of other fish genera and species available in GenBank. The barcoding result (DNA sequencing) showed that it fit with C. kais. We conclude the results of the current study confirm that the validity of the morphological diagnosis of C. kais, using DNA sequencing analysis.

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Published

2023-12-18

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Articles